Long Term Effect of Biodegradable Polymer on Oxidative Stress and Genotoxicity

Oxidative stress and genotoxicity induced by intraperitoneally implanted polycaprolactone (PCL) were evaluated in present study. In an effort to assess oxidative stress induced liver injury, we measured hematological indices, biochemical parameters of liver function and levels of oxidative stress biomarkers such as lipid peroxidation (LPO), 8hydroxy-2’deoxy guanosine (8-OHdG), reduced glutathione(GSH) and glutathione reductase(GR) after three months as well as twelve months of implantation. Another objective of the study was to analyze chromosomal aberrations induced by the extract of polycaprolactone in human peripheral venous blood lymphocytes using GTG banding technique and karyotyping of human chromosomes. No significant changes were displayed by the experimental animals in the hematological parameters. Furthermore, liver function were normal as evidenced by the activities of aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), serum bilirubin, cholesterol, triglyceride and albumin. Oxidative stress statuses were comparable with the control animals after 3 and 12 months of intraperitoneal implantation. Karyotype of human chromosomes were found to be normal indicating the nongenotoxic effects of polycaprolactone. Hence it can be concluded that polycaprolactone used for this study is non-toxic at the molecular level, under the environmental conditions monitored.